Expression Data

Here is a description of the custom expression microarrays developed by the Arborea II and SMarTForests as well as a brief description of the experiments in which they were used.

Microarray
Description
Accession
Platform
Publication
Spruce11k cDNA microarray Spotted cDNA, 10,400 nonredundant sequences, printed as double, side-by-side spots GPL10754 GEO Pavy et al. 2008
Spruce32k Oligo microarray Spotted oligonucleotide, 70 bp, 31,604 White and Sitka spruce gene-probes GPL15033 GEO Raherison et al. 2012

 

Microarray Experiments

Title
Array format
Accession
Platform
Publication
Analysis of recacitrance to induction of somatic embryogenesis, shoot primordia, adult trees, Picea glauca Spruce32k GSE46977 GEO Rutledge et al. 2013
Evaluation of differential expression in the vascular transcriptome among Picea spp. Spruce32k GSE35922 GEO Raherison et al. 2012
Interspecies Pinaceae comparison experiment (7 spp.) Spruce32k GSE35847 GEO Raherison et al. 2012
PiceaGenExpress database of transcription profiles (8 tissues) Spruce32k GSE35624 GEO Raherison et al. 2012
Direct measurement of heritable gene expression within single individuals from wild populations Spruce32k GSE35337 GEO Verta et al. 2013
Transcription profiling of Picea glauca xylem and phloem Spruce11k E-MEXP-1489 ArrayExpress Pavy et al. 2008
Molecular events of apical bud formation in white spruce Spruce11k GSE23519 GEO El Kayal et al. 2011
Integrated transcriptomic and proteomic profiling of white spruce stems during the transition from active growth to dormancy Spruce11k GSE31090 GEO Galindo González et al. 2012

 

RNAseq gene expression

RNAseq
Description
Accession
Platform
Publication
Bark Bark tissue from PG29 was extracted for total RNA and sequenced SRX318118 Illumina Hi Seq Warren et al. 2015
Flushing Bud Flushing bud needles from PG29 were extracted for total RNA and sequenced SRX318119 Illumina Hi Seq Warren et al. 2015
Mature Needle Mature needles from PG29 were extracted for total RNA and sequenced SRX318120 Illumina Hi Seq Warren et al. 2015
Xylem Xylem tissues from PG29 were extracted for total RNA and sequenced SRX318121 Illumina Hi Seq Warren et al. 2015
Young buds Young buds from PG29 were extracted for total RNA and sequenced SRX318122 Illumina Hi Seq Warren et al. 2015
Seedling Seedlings from PG29 were extracted for total RNA and sequenced SRX318123 Illumina Hi Seq Warren et al. 2015
Embryo Embryos from PG29 were extracted for total RNA and sequenced SRX318124 Illumina Hi Seq Warren et al. 2015
Megagametophytes Megagametophytes from PG29 were extracted for total RNA and sequenced SRX318125 Illumina Hi Seq Warren et al. 2015